Device

Part:BBa_K1783001:Design

Designed by: Iowis Zhu   Group: iGEM15_UMaryland   (2015-06-30)

Hok/Sok Type I Toxin/Antitoxin Cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ordered a g-block of the Hok/Sok sequence from IDT with extensions into pSB1C3. We then Gibson assembled the g-block into the backbone using NEB HiFi mix. We confirmed successful cloning via sequencing. Note: Further PCR amplification of this construct is difficult due to high inherent secondary structure of the Hok/Sok cassette.

Source

K-12 plasmid pDM0133

References

Source from BLAST search